The large-scale CELEBRATION 2000 seismic experiment investigated the velocity structure of the crust and upper mantle between western portion of the East European Craton (EEC) and the eastern Alps. This area comprises: the Trans-European Suture Zone, the Carpathian Mountains, the Pannonian Basin and the Bohemian Massif. This experiment included 147 chemical shots recorded by 1230 seismic stations during two deployments. Good quality data along 16 main and a few additional profiles were recorded. One of them, profile CEL03, was located in southeastern Poland and was laid out as a prolongation of the TTZ profile performed in 1993. This paper focuses on the joint interpretation of seismic data along the NW–SE trending TTZ–CEL03 transect, located in the central portion of the Trans-European Suture Zone. First arrivals and later phases of waves reflected/refracted in the crust and upper mantle were interpreted using two-dimensional tomographic inversion and ray-tracing techniques. This modelling established a 2-D (quasi 3-D) P-wave velocity lithospheric model. Four crustal units were identified along the transect. From northwest to southeast, thickness of the crust varies from 35 km in the Pomeranian Unit (NW) to ∼40 km in the Kuiavian Unit, to ∼50 km in the Radom–Łysogóry Unit and again to ∼43 km in the Narol Unit (SE). The first two units are thought to be proximal terranes detached from the EEC farther to the southeast and re-accreted to the edge of the EEC during the Early Palaeozoic. The origin of the remaining two units is a matter of dispute: they are either portions of the EEC or other proximal terranes. In the area of the Polish Basin (first two units), the P-wave velocity is very low (Vp < 6.1 km/s) down to depths of 15–20 km indicating that a very thick sedimentary and possibly volcanic rock sequence, whose lower portion may be metamorphosed, is present. The velocity beneath the Moho was found to be rather high, being 8.25 km/s in the northwestern portion of the transect, 8.4 km/s in the central sector, and 8.1 km/s in the southeastern sector.